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anti cbp  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti cbp
    Anti Cbp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 270 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cbp/CBP+Rabbit+mAb/pm41904119-271-19-21
    Average 96 stars, based on 270 article reviews
    anti cbp - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: EP300 deficiency leads to chronic replication stress mediated by defective replication fork protection.
    Article Snippet: .. Antibodies for western blot included anti-Vinculin (Sigma Aldrich – V9131, 1:1000), anti-pH2AX (Cell Signaling – 9718T, 1:1000), anti-pCHK1 (s317) (Cell signaling –12302, 1:1000) , anti-pRPA (ser4, ser8) (Bethyl Lab A300-245A-M, 1:1000), anti-CHK1 (Cell Signaling – 2360, 1:1000), anti-RPA (Bethyl – A300-244A, 1:1000), antiH3 (Cell Signaling – 12648T, 1:5000), anti-H3K27ac (Cell Signaling 8173T, 1:1000), anti-EP300 (ABCAM- ab10485, Cell Signaling- 70088 1:1000), anti-BRCA1 (EDM Millipore – 07434) , antiBRCA2 (EDM Millipore – OP95, 1:1000), anti-rad51 (Abcam – ab63801, CosmobioUSA- Bam70-002-EX, 1:500), phospho-ATR (Cell Signaling, Cat#30632, 1:2000), Anti-CBP (Cell Signaling Technology, Cat# 7389, 1:500). ..

    Article Title: Targeting MYC in T-Cell lymphoma via epigenetic modulation with a small-molecule inhibitor.
    Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS The primary antibodies used in our study for western blotting were anti-MYC (1:1000; CST, Cat# 9402), anti-YB-1 (1:1000; Abcam, Cat# ab76149), anti-p53 (1:1000; Abcam, Cat# ab26), anti-E2F1 (1:1000; Proteintech, Cat# 66515-1-IG), Anti-H3K4me3 (1:1000; PTM BIO, Cat# PTM-613), Anti-H3K9me3 (1:1000; PTM BIO, Cat# PTM-616), Anti-H3K27me3 (1:1000; PTM BIO, Cat# PTM-647RM), Anti-H3K27ac (1:1000; PTM BIO, Cat# PTM-116RM), Anti-H3K9ac (1:1000; PTM BIO, Cat# PTM-112RM), Anti-H3K9la (1:1000; PTM BIO, Cat# PTM-1419RM), Anti-H3K14la (1:1000; PTM BIO, Cat# PTM-1414RM), Anti-H3K18la (1:1000; PTM BIO, Cat# PTM1406RM), Anti-H4K12la (1:1000; PTM BIO, Cat# PTM-1411RM), Anti-H4K16la (1:1000; PTM BIO, Cat# PTM-1417RM), Anti-Histone H3 (1:1000; CST, Cat# 9715), Anti-Histone H4 (1:1000; PTM BIO, Cat# PTM-1015RM), anti-Flag-Tag (1:1000; CST, Cat# 8146), antiMyc-tag (1:1000; CST, Cat# 2278), anti-HA-Tag (1:1000; CST, Cat# 3724), anti-p300 (1:1000; Abcam, Cat# ab275378), anti-CBP (1:1000; CST, Cat# 7389), anti-KAT8 (1:1000; CST, Cat# 46862), anti-AARS1 (1:1000; Proteintech, Cat# 67909-1-IG), anti-AARS2 (1:1000; Proteintech, Cat# 22696-1-IG), anti-UCHL3 (1:1000; Abcam, Cat# ab241490), anti-α-tubulin (1:2000; Proteintech, Cat# 11224-1-AP). ..

    Isolation:

    Article Title: CBP phosphorylation maintains intestinal homeostasis by supporting the stem cell niche through versican.
    Article Snippet: CUT&RUN assay for CBP and p53 enrichments at the p21 promoter The CUT&RUN assay was performed using the CUT&RUN Assay Kit (Cell Signaling Technology, #86652) according to the manufacturer’s protocol, with minor modifications. .. Briefly, 1-3 × 105 freshly isolated colonic crypt cells were fixed with 0.1% formaldehyde, permeabilized with digitonin-containing buffer, and incubated with anti-CBP (1:50; Cell Signaling Technology, #7389) and anti-p53 (1:50; Cell Signaling Technology, #2524) antibodies to capture CBP-bound and p53-bound chromatin, respectively. ..

    Incubation:

    Article Title: CBP phosphorylation maintains intestinal homeostasis by supporting the stem cell niche through versican.
    Article Snippet: CUT&RUN assay for CBP and p53 enrichments at the p21 promoter The CUT&RUN assay was performed using the CUT&RUN Assay Kit (Cell Signaling Technology, #86652) according to the manufacturer’s protocol, with minor modifications. .. Briefly, 1-3 × 105 freshly isolated colonic crypt cells were fixed with 0.1% formaldehyde, permeabilized with digitonin-containing buffer, and incubated with anti-CBP (1:50; Cell Signaling Technology, #7389) and anti-p53 (1:50; Cell Signaling Technology, #2524) antibodies to capture CBP-bound and p53-bound chromatin, respectively. ..



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    Image Search Results


    Naïve IgM + IgD + B cells from Aicda Cre Ogt +/+ and Aidca Cre Ogt fl/fl mice spleen ( n = 3, A–C) or Aicda Cre Tet2 +/+ and Aidca Cre Tet2 fl/fl mice spleen ( n = 3, B and G) were cultured for 72 h or 96 h upon application of GC-like cell-inducing stimuli. (A) Left, CD19 + IgG1 + , CD19 + IgG3 + or CD19 + IgA + B cells and CD19 + CD138 + plasmablasts. Right, intracellular AID and BLIMP-1 expression in B cells stimulated with LPS plus IL-4 after 96 h microculture. (B) Methylated and hydroxymethylated DNA levels in promoter and enhancer regions of Aicda and Prdm1 gene loci in Aicda Cre Tet2 +/+ , Aicda Cre Tet2 fl/fl (top), Aicda Cre Ogt +/+ , and Aicda Cre Ogt fl/fl (bottom) cells after 72 h microculture, as analyzed by MeDIP-qPCR and hMeDIP-qPCR. (C) Expression of TET2 and O -GlcNAcylated TET2 in Aidca Cre Ogt +/+ and Aidca Cre Ogt fl/fl cells after 96 h microculture. (D) Naïve IgM + IgD + B cells from C57BL/6 ( n = 3) mice spleen or healthy humans ( n = 3) were cultured for 0, 24, 48, 72, and 96 h upon stimulation with LPS plus IL-4 or CpG (ODN 2395) plus huIL-2, huIL-4, and huIL-21, respectively. Expression of TET2 and O -GlcNAcylated TET2 in mouse and human B cells at the indicated time points. (E) TET2 O -GlcNAcylation sites in mouse B cells, as predicted by YinOYang and O -GlcNAcPRED-DL. (F) Naïve IgM + IgD + B cells from C57BL/6 mice ( n = 3) spleen were cultured for 72 h upon stimulation with LPS plus IL-4. TET2 or OGT interaction with TET2, OGT, PROSER1, HDAC1, and CREBBP in cell nuclei, as analyzed by specific immunoblots. (G) Aidca Cre Tet2 +/+ and Aidca Cre Tet2 fl/fl B cell OGT (left) and O -GlcNAc H2B-S112 (right) levels after 72 h microculture, as expressed in promoter and enhancer regions of Aicda and Prdm1 gene loci, as analyzed by ChIP-qPCR. Statistical significance (B and G) was assessed using two-sided Student’s unpaired t test (** p < 0.01, *** p < 0.001).

    Journal: Cell reports

    Article Title: B cell TET2 recruits OGT for nuclear TET2 and H2B O -GlcNAcylation to drive AID and BLIMP-1 expression and maturation of the antibody response

    doi: 10.1016/j.celrep.2026.117370

    Figure Lengend Snippet: Naïve IgM + IgD + B cells from Aicda Cre Ogt +/+ and Aidca Cre Ogt fl/fl mice spleen ( n = 3, A–C) or Aicda Cre Tet2 +/+ and Aidca Cre Tet2 fl/fl mice spleen ( n = 3, B and G) were cultured for 72 h or 96 h upon application of GC-like cell-inducing stimuli. (A) Left, CD19 + IgG1 + , CD19 + IgG3 + or CD19 + IgA + B cells and CD19 + CD138 + plasmablasts. Right, intracellular AID and BLIMP-1 expression in B cells stimulated with LPS plus IL-4 after 96 h microculture. (B) Methylated and hydroxymethylated DNA levels in promoter and enhancer regions of Aicda and Prdm1 gene loci in Aicda Cre Tet2 +/+ , Aicda Cre Tet2 fl/fl (top), Aicda Cre Ogt +/+ , and Aicda Cre Ogt fl/fl (bottom) cells after 72 h microculture, as analyzed by MeDIP-qPCR and hMeDIP-qPCR. (C) Expression of TET2 and O -GlcNAcylated TET2 in Aidca Cre Ogt +/+ and Aidca Cre Ogt fl/fl cells after 96 h microculture. (D) Naïve IgM + IgD + B cells from C57BL/6 ( n = 3) mice spleen or healthy humans ( n = 3) were cultured for 0, 24, 48, 72, and 96 h upon stimulation with LPS plus IL-4 or CpG (ODN 2395) plus huIL-2, huIL-4, and huIL-21, respectively. Expression of TET2 and O -GlcNAcylated TET2 in mouse and human B cells at the indicated time points. (E) TET2 O -GlcNAcylation sites in mouse B cells, as predicted by YinOYang and O -GlcNAcPRED-DL. (F) Naïve IgM + IgD + B cells from C57BL/6 mice ( n = 3) spleen were cultured for 72 h upon stimulation with LPS plus IL-4. TET2 or OGT interaction with TET2, OGT, PROSER1, HDAC1, and CREBBP in cell nuclei, as analyzed by specific immunoblots. (G) Aidca Cre Tet2 +/+ and Aidca Cre Tet2 fl/fl B cell OGT (left) and O -GlcNAc H2B-S112 (right) levels after 72 h microculture, as expressed in promoter and enhancer regions of Aicda and Prdm1 gene loci, as analyzed by ChIP-qPCR. Statistical significance (B and G) was assessed using two-sided Student’s unpaired t test (** p < 0.01, *** p < 0.001).

    Article Snippet: anti-mo/hu-CREBBP mAb , ABclonal , Cat. # A26780.

    Techniques: Cell Culture, Expressing, Methylation, Methylated DNA Immunoprecipitation, Western Blot, ChIP-qPCR